ࡱ> 130bjbj 4hh((vvvvv  "Hvvvvv:,Q@-SF 0R##Q#vQ@#( <:Effects of Voluntary Running on Spatial Memory and Mature Brain-Derived Neurotrophic Factor Expression in Mice Hippocampus after Status Epilepticus Induced by Pilocarpine Sartori MSc, Cesar1; Pelgio MSc, Fernanda1; Ferrari PhD, Elenice1; Valentinuzzi PhD, Vernica1; Nascimento, MSc Andr1; Rogrio PhD, Fbio1; Teixeira PhD, Simone2; Muscar PhD, Marcelo2; Langone PhD, Francesco1 1 Department of Physiology & Biophysics, Institute of Biology, State University of Campinas 2 Department of Pharmacology, Institute of Biomedical Sciences, University of So Paulo Rationale: Voluntary physical activity improves memory and learning ability in rodents whereas status epilepticus has been associated with memory impairment. Physical activity and seizures have been associated with enhanced hippocampal expression of BDNF, indicating that this protein may have a dual role in epilepsy. The influence of voluntary physical activity on memory and BDNF expression has been poorly studied in experimental models of epilepsy. Objective: In this work, we have investigated the effect of voluntary physical activity on memory and BDNF expression in mice with pilocarpine-induced epilepsy. Methods: Male Swiss mice were assigned to four experimental groups: pilocarpine sedentary (PS), pilocarpine runners (PR), saline sedentary (SS) and saline runners (SR). Two days after pilocarpine-induced status epilepticus, the affected mice (PR) and their running controls (SR) were housed with access to a running wheel for 28 days. After that, the spatial memory and the expression of the precursor and mature forms of hippocampal BDNF were assessed. Results: . PR mice performed better than PS mice in the water maze test. In addition, PR mice had a higher amount of mature BDNF (14 kDa) relative to the total BDNF (14 kDa + 28 kDa + 32 kDa forms) content when compared with PS mice. In addition, PR mice had a higher amount of mature BDNF (14 kDa) relative to the total BDNF (14 kDa + 28 kDa + 32 kDa forms) content when compared with PS mice. Conclusion: These results show that voluntary physical activity improved the spatial memory and increased the hippocampal content of mature BDNF of mice with pilocarpine-induced status epilepticus.      ) * . @ A E Y Z [ \ ^ r s u v w G J յh'Q:hjkmH sH h'Q:hu\h'Q:hjkH*mH sH h'Q:hjkmHsHh'Q:humHsHh'Q:huH*mHsHh'Q:mHsHh'Q:hjkH*mHsHh'Q:h7 mHsHh'Q:hsmHsHh'Q:hq`5PJh'Q:hs5PJ0 G H J  {gdjkgd'Q:gdugdsgd'Q:gdq`m$J T V     {ɽɶ h'Q:hh'Q:h'Q:hjk5 h'Q:hjkh'Q:hjk56h'Q:hsOJQJ^Jh'Q:hjkmH sH h'Q:hjk56mH sH 21h:pF/ =!"n#n$n% ^ 666666666vvvvvvvvv666666>6666666666666666666666666666666666666666666666666hH6666666666666666666666666666666666666666666666666666666666666666662 0@P`p2( 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p8XV~_HmHnHsHtH@`@ NormalCJ_HaJmH sH tH XX jkTtulo 1$<@&5CJ KH OJQJ\^JaJ >A > Fonte parg. padroTiT  Tabela normal4 l4a ,k , Sem lista R`R q`Sem EspaamentoCJ_HaJmH sH tH PK![Content_Types].xmlj0Eжr(΢Iw},-j4 wP-t#bΙ{UTU^hd}㨫)*1P' ^W0)T9<l#$yi};~@(Hu* Dנz/0ǰ $ X3aZ,D0j~3߶b~i>3\`?/[G\!-Rk.sԻ..a濭?PK!֧6 _rels/.relsj0 }Q%v/C/}(h"O = C?hv=Ʌ%[xp{۵_Pѣ<1H0ORBdJE4b$q_6LR7`0̞O,En7Lib/SeеPK!kytheme/theme/themeManager.xml M @}w7c(EbˮCAǠҟ7՛K Y, e.|,H,lxɴIsQ}#Ր ֵ+!,^$j=GW)E+& 8PK!.atheme/theme/theme1.xmlYMoE#F{om'vGuرhF[xw;jf7q7J\ʉ("/z'4IA!>Ǽ3|^>5.=D4 ;ޭªIOHǛ]YxME$&;^TVIS 1V(Z Ym^_Ř&Jp lG@nN&'zξ@F^j$K_PA!&gǬへ=!n>^mr eDLC[OF{KFDžƠپY7q~o >ku)lVݜg d.[/_^йv[LԀ~Xrd|8xR{ (b4[@2l z "&'?>xpxGȡIXzg=2>ϫPCsu=o<.G4& h`9Q"LI(q }93̲8ztzH0SE+$_b9rQkZVͣiV 2n*=8OSyZ:"⨹ppH~_/PŴ%#:viNEcˬfۨY՛dEBU`V0ǍWTḊǬXEUJg/RAC8D*-Um6]Ptuyz*&Q܃h*6w+D?CprloSnpJoBӁc3 chϿ~TYok#ހ=pGn=wOikZoiBs͜zLPƆjui&e E0EMl8;|͚ 64HpU0)L O3 e:(xfä)Hy`r~B(ؘ-'4g\вfpZa˗2`khN-aT3ΑV \4  o`v/] f$~p p@ic0As\ @THNZIZ[}i RY\qy$JyϣH9\,AZjyiǛ)D]n|%lڟX̦l熹EЀ > 6ljWY DK/eby_膖L&W`VcJT14fS!:UJ0A?y6Xg1K#[]y%[BTRlwvSLɟ)4.Xt|zx\CJ#Lw@,e_}֜aN}jHP؏T$فdfl,YdTI]Zd+zoPnI hYC=!kk|l1Qn6MBŊ]|-_Ǭf^ Mθڎ`R+Wh1,Q >H *:[䠙A@V_ .ap64+lt^7st G5;Mb8s9x<ڮjI~11qM2%M2K94uo%PK! ѐ'theme/theme/_rels/themeManager.xml.relsM 0wooӺ&݈Э5 6?$Q ,.aic21h:qm@RN;d`o7gK(M&$R(.1r'JЊT8V"AȻHu}|$b{P8g/]QAsم(#L[PK-![Content_Types].xmlPK-!֧6 +_rels/.relsPK-!kytheme/theme/themeManager.xmlPK-!.atheme/theme/theme1.xmlPK-! ѐ' theme/theme/_rels/themeManager.xml.relsPK]  J   l,b$fӏnX)l3S_LV@0(  B S  ?HT #^ew~JT+6lwZe$'-069vFJ3{  *.AEZ[\^suvw@ + xVv8\h^`OJQJo(hHh^`OJQJ^Jo(hHohp^p`OJQJo(hHh@ ^@ `OJQJo(hHh^`OJQJ^Jo(hHoh^`OJQJo(hHh^`OJQJo(hHh^`OJQJ^Jo(hHohP^P`OJQJo(hHh^`OJQJo(hHh^`OJQJ^Jo(hHohp^p`OJQJo(hHh@ ^@ `OJQJo(hHh^`OJQJ^Jo(hHoh^`OJQJo(hHh^`OJQJo(hHh^`OJQJ^Jo(hHohP^P`OJQJo(hH@ +Vv8  7 'Q:q`!dk55{jkusF@@UnknownG*Ax Times New Roman5Symbol3. *Cx ArialI. ??Arial Unicode MSCTimesNewRoman?= *Cx Courier New;WingdingsA BCambria Math"qh+Ɔ+Ɔauau!n24 2qHX ?!dk2!xx}TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE Li Li Min Francesco  Oh+'0x, @L l x TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE Li Li MinNormal Francesco2Microsoft Office Word@V@4y-@4y-au՜.+,0p hp  O FCM UNICAMP TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE TITLE Ttulo !"#$%&')*+,-./2Root Entry F.-41Table $WordDocument4SummaryInformation( DocumentSummaryInformation8(CompObj}  F+Documento do Microsoft Office Word 97-2003 MSWordDocWord.Document.89q